Dr. Praveen Chandrasekharan
Center for Developmental Biology of the Lung

Research

where the real work gets done

Center for Developmental Biology of the Lung

A dedicated laboratory space at the University of Buffalo, the State University of New York, features a surgical suite, lamb intensive are unit, isolated tissue baths, and stations for molecular biology, Western blot and RT^2 PCR analysis. Our experience in laboratory models include: the premature ventilated lamb, the fetal sheep ductal ligation-induced PPHN model, a sheep congenital diaphragmatic hernia model, the neonatal rabbit hyperoxia-induced fibrosis, and various transgenic mice lines.


 Genomic cluster obtained using 10x genomics on a preterm ovine model

Genomic cluster obtained using 10x genomics on a preterm ovine model

Genomics & Bioinformatics Core Facility

This facility is the first to provide next-generation sequencing in Western New York. The 10x Genomics Chromium platform specializes in single-cell RNA-Seq, and whole genome linked libraries.


 Fetal heart anatomy

Fetal heart anatomy

Intrathoracic surgery

Our staff specialize in the placement of invasive monitoring equipment (Transonic Systems, Inc.) to aid in our understanding of the complex physiology of transition at birth.


 Biopac Acknowlege software recording of in-vivo monitoring

Biopac Acknowlege software recording of in-vivo monitoring

In-vivo Monitoring

Acknowledge software (Biopac Systems, Inc.) allows us to to record real time data and analyze changes in blood flow and blood pressure in response to various interventions.


 
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The Perinatal Asphyxiated Lamb Model

A model for newborn resuscitation

We recently published a written manuscript with supplemental video to describe the unique surgical technique performed in our laboratory. The manuscript is open access; a video clip can viewed here.

Elizabeth Doughman, (Editor-in-Chief, ALN Magazine) featured our publication in her online editorial, “Lamb Model Could Help Human Babies Breathe Better.”

 

Grant Activity

American Academy of Pediatrics – Neonatal Resuscitation Program Young Investigator Grant 

  • Optimizing Chest Compressions Targeting Gas Exchange and Hemodynamics in a Transitional Cardiac Arrest Model

  • January 2018- Current

  • The objective is to assess the optimal method of delivering chest compressions based on gas exchange leading to successful return of spontaneous circulation

  • Primary Investigator: Dr. Chandrasekharan

University at Buffalo Jacobs School of Medicine and Biomedical Sciences: Buswell Research Grant  

  • Placental Transfusion in Preterm Resuscitation

  • July 2015-June 2018

  • The research looks at various modes of placental transfusion such as delayed cord clamping, umbilical cord milking, umbilical cord milking in a preterm model with perinatal asphyxia. 

  • Primary Investigator: Dr. Chandrasekharan

Neonatal Research Network: University at Buffalo/University of Rochester

  • April 2018- March 2022

  • 18 neonatal perinatal centers are involved in this network and conduct multicenter randomized controlled trials along with data sharing to improve the quality of neonatal care

  • Co-Investigator: Dr. Chandrasekharan

Neonatal Resuscitation Program

  • Flush Volume and Epinephrine Pharmacokinetics

  • November 2017- Current

  • To study the effect of using various flush volumes on epinephrine pharmacokinetics and resuscitation in a neonatal model

  • Primary Investigator: Dr. Chandrasekharan


Perspectives from a Research Technician

In the lab, our most exciting days begin with anesthesia of a pregnant ewe, performing a cesarean section, calibration of our monitoring equipment, and intra-thoracic surgery on a fetal lamb, or two. After conducting the experiment, tissues are harvested and banked for future analysis. The day ends with decontamination of the surgical suite, and backing up the precious in vivo data that was recorded. The next morning we may prepare for a tissue bath study. This involves microdissection, drug dilutions, and careful placement of airway or vessels on force transducers. Meanwhile, assays are run, cells are in culture, and tissues are prepared for immunohistochemistry Later, we combine measurements taken with Biopac Systems, Nonin Sensmart, Philips NM3, and the hand written record of the experiment into one elaborate Microsoft Excel sheet. Then, the statistics begin, the figures are generated, and we compile our results for presentations, manuscripts or grant applications.


Research is creating new knowledge.
— Neil Armstrong